Select Page
Dr. Harold Aukema

Dr. Harold Aukema

Principal Investigator
Nutritional Lipidomics, Canadian Centre for Agri-food Research In Health and Medicine

Professor
Human Nutritional Sciences, University of Manitoba

Research Focus

Our laboratory has been examining the role of prostanoid related enzymes and prostanoid production in normal and diseased kidneys. These findings are relevant to the questions surrounding the nephrotoxicity of selective cyclooxygenase inhibitors and the role of these fatty acid derived mediators in renal physiology. Our current work is demonstrating that early dietary interventions (such as with fish oil and soy protein) alter the levels these products. We have developed comprehensive lipidomic analysis of oxylipins in our laboratory and expanded this work from prostanoids to include over 100 oxylipins. This analysis has led to the discovery of novel compounds in response to dietary interventions (e.g. unique oxylipins produced from dietary flax and canola oil feeding) and unique oxylipins patterns in several types of renal diseases.

The laboratory also examines the roles of dietary interventions in the early progression of kidney disease. This includes nutritional, functional food and pharmacological interventions in several types of kidney disease, including polycystic kidney disease (using conditional knockout models), obesity related renal disorders and pediatric forms of renal cyst disease. These studies are being carried out in collaboration with investigators at Fujita Health University in Japan. As an example of the impact of these studies, some patient organizations now include dietary recommendations to patients with polycystic kidney disease for reduction in dietary protein, and substituting plant for animal proteins.

Our work on the effects of dietary proteins on normal kidney physiology has implications for the dietary recommendations for dietary protein. Findings in both rat and pig models suggest that, despite the potential benefit of high protein diets on body composition, long-term intakes of protein at the upper limit of the dietary recommendations may compromise renal health. We are currently examining the effects of different types of protein in high protein diets in the context of obesity, as renal damage in obesity may predispose the obese to further damage by high levels of dietary protein. Different protein sources at high levels of intake may have differing effects, whether positive or negative.

For more information, contact:

Dr. Harold Aukema
Fax. (204) 237-2289
Email. harold.aukema@umanitoba.ca

Peer-Reviewed Papers

Mudryj AN, Yu N, Hartman TJ, Mitchell DC, Lawrence FR, Aukema HM. Pulse consumption in canadian adults influences nutrient intakes. Br J Nutr 108: S27-36

Yoshihara D, Kugita M, Yamaguchi T, Aukema HM, Kurahashi H, Morita M, Hiki Y, Calvet JP, Wallace D, Toyohara T, Abe T, Nagao S. Global gene expression profiling in PPAR-γ agonist-treated kidneys in an orthologous rat model of human autosomal recessive polycystic kidney disease. PPAR Res:695898

Wakefield AP, Ogborn MR, Ibrahim N, Aukema HM. A dietary conjugated linoleic acid treatment which slows renal disease progression alters renal cyclooxygenase-2 derived prostanoids in the Han:SPRD-cy rat. J Nutr Biochem 23:908-914

Liu X, Joseph SV, Wakefield AP, Aukema HM, Jones PJH. High dose trans-10,cis-12 CLA increases lean body mass in hamsters, but elevates levels of plasma lipids and liver enzyme biomarkers. Lipids 47:39-46

Caligiuri, S.P.B.,Dunthorne, K., Gregoire, M., Wu, Y., Winter, T., Gauthier, J., Taylor, C., Blydt-Hansen, T., Zahradka, P. & Aukema, H. Novel alpha-linolenic acid (ALA) derived metabolites correlate with the renoprotective effects of ALA rich dietary oils in diet-induced obesity. Canadian Nutrition Society, Guelph.

Invited Presentations

Importance of alpha-linolenic acid in canola. Presented to Chinese delegation of canola plant breeders and agronomists. University of Manitoba, Winnipeg

Abstracts and Presentations

Kugita M, Yoshihara D, Horie S, Aukema HM, Yamaguchi T, Nagao S. Telmisartin ameliorates fibrocystic liver disease in PCK rats, an orthologous model of human ARPKD. Molecular Biology Society of Japan

Caligiuri SPB, Dunthorne K, Grégoire M, Wu Y, Winter T, Gauthier J, Taylor CG, Blydt-Hansen T, Zahradka P, Aukema HM. Examination of the renal eicosanoid lipidome reveals potential renoprotective effects of alpha linolenic acid metabolites in a model of early obesity-related glomerulopathy. 14th Annual Institute for Cardiovascular Sciences Awards Program

Yoshihara D, Kugita M, Horie S, Aukema HM, Yamaguchi T, and Nagao S. Telmisartan ameliorates fibrocystic liver disease in an orthologous rat model of human ARPKD. Annual Meeting of the American Society of Nephrology

Caligiuri SPB, Dunthorne K, Grégoire M, Wu Y, Winter T, Gauthier J, Taylor CG, Blydt-Hansen T, Zahradka P, Aukema HM. Examination of the renal eicosanoid lipidome reveals potential renoprotective effects of alpha linolenic acid metabolites in a model of obesity-related glomerulopathy. Functional Food and Natural Health Product Graduate Student Symposium, Winnipeg

Ibrahim N, Jia Y, Devassy J, Gauthier J, Yamaguchi T, Aukema HM. Differential effects of dietary fish oil and soy protein on renal disease and eicosanoids in chronic kidney disease. Functional Food and Natural Health Product Graduate Student Symposium, Winnipeg

Devassy JG, Wojcik J, Taylor CG, Zahradka P, Aukema HM. Influence of the source of protein on effect of high protein diets on kidney in the obese rat. Functional Food and Natural Health Product Graduate Student Symposium, Winnipeg

Caligiuri SPB, Dunthorne K, Grégoire M, Wu Y, Winter T, Gauthier J, Taylor CG, Blydt-Hansen T, Zahradka P, Aukema HM. Examination of the Renal Eicosanoid Lipidome Reveals Potential Renoprotective Effects of Alpha Linolenic Acid Metabolites in a Model of Early Obesity-Related Glomerulopathy, Canadian Student Health Research Forum, Winnipeg

Caligiuri SPB, Dunthorne K, Grégoire M, Wu Y, Winter T, Gauthier J, Taylor CG, Blydt-Hansen T, Zahradka P, Aukema HM. Potential renoprotective effects of novel eicosanoids produced in diet-induced obese rats given α-linolenic acid rich diets. International Society for the Study of Fatty Acids and Lipids, Vancouver

Ibrahim N, Jia Y, Devassy J, Gauthier J, Yamaguchi T, Aukema HM. Differential effects of dietary fish oil and soy protein on renal disease and eicosanoids in chronic kidney disease. International Society for the Study of Fatty Acids and Lipids, Vancouver

Caligiuri SPB, Dunthorne K, Grégoire M, Wu Y, Winter T, Gauthier J, Taylor CG, Blydt-Hansen T, Zahradka P, Aukema HM. Novel alpha-linolenic acid (ALA) derived metabolites correlate with the renoprotective effects of ALA rich dietary oils in diet-induced obesity. Appl Physiol Nutr Metab 37: 566 (CNS Nestle Graduate Student Award)

Ibrahim N, Devassy J, Jia Y, Gauthier J, Yamaguchi T, Aukema HM. Dietary soy protein slows disease progression and has specific effects on renal eicosanoids in cystic kidney disease. Appl Physiol Nutr Metab 37: 576,

Caligiuri SPB, Dunthorne K, Grégoire M, Wu Y, Winter T, Gauthier J, Taylor CG, Blydt-Hansen T, Zahradka P, Aukema HM. Renoprotective effects of ALA enriched dietary oils in early obesity-related glomerulopathy. Dietitians of Canada Research Day, Manitoba

Caligiuri, S.P.B.,Dunthorne, K., Gregoire, M., Wu, Y., Winter, T., Gauthier, J., Taylor, C., Blydt-Hansen, T., Zahradka, P. & Aukema, H. Examination of the renal eicosanoid lipidome reveals potential renoprotective effects of alpha linolenic acid metabolites in a model of early obesity-related glomerulopathy. Canadian Student Health Research Forum, Winnipeg.


View on Mendeley

View on Scopus

orcid-hero-logo

University of Manitoba Merit Award for Excellence in Research, Teaching and Service

Studenship, Nestle Graduate Student Competition at the Annual Meeting of the Canadian Nutrition Society, Vancouver – Stephanie Caligiuri

Canadian Institutes of Health Research
Effect of dietary interventions on disease progression in orthologous models of polycystic kidney disease
$118,156

Natural Sciences and Engineering Research Council of Canada
Dietary effects on renal eicosanoid metabolism
$44,400

Agri-Food Research Development Initiative (ARDI)
Biological effects of alpha-linolenic acid metabolites
$28,000

Media

Life In the Lab